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Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis

Objective: To explore the application of self-made distractors to construct a unilateral DO model of rabbit mandible and evaluate the microstructure of bone specimens. Method: Designing a linear dual arm distractor and performing DO implantation surgery on 5 rabbits under local anesthesia. Stretching the mandible at rate of 1.0mm/day, 2 times/day for 10 days. After fixation for 3 months, taking mandibular specimens for gross observation, Micro-CT imaging and HE histological examination to analyze the bone density, maturity and three-dimensional structure of the new bone. Result: Except for one animal that died of unknown cause, the right mandibular body of the other animals was extended by about 10mm. The traction gap is completely filled with bright and red new bone with clear contours; Micro-CT reconstruction of three-dimensional bone images clearly shows high bone density in the gaps, with trabeculae arranging along the direction of distraction; HE staining shows that the bone trabeculae were connected into woven and layered bones, with a large number of concentric arranged bone plates surrounding the central canal to form the Haversian system, and osteoblasts proliferated actively. Conclusion: The use of Micro-CT and HE staining proves the success of the unilateral DO model of rabbit mandible using a self-made distractor, providing animal experiment for various biological factors and drug assisted methods to promote efficient DO in the later stage, and for its safe and effective clinical application.

Distraction Ssteogenesis, Mandible, Micro-CT, Three-Dimensional Structure

APA Style

Li Peng, Xiao Xin, He Chuying, Song Jing, Long Jie. (2023). Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. International Journal of Dental Medicine, 9(1), 23-27.

ACS Style

Li Peng; Xiao Xin; He Chuying; Song Jing; Long Jie. Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. Int. J. Dent. Med. 2023, 9(1), 23-27. doi: 10.11648/j.ijdm.20230901.15

AMA Style

Li Peng, Xiao Xin, He Chuying, Song Jing, Long Jie. Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. Int J Dent Med. 2023;9(1):23-27. doi: 10.11648/j.ijdm.20230901.15

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. ILIZAROV, G, A, et al. The Replacement of Long Tubular Bone Defects by Lengthening Distraction Osteotomy of One of the Fragments [J]. Clinical Orthopaedics and Related Research, 1992.
2. Ilizarov GA. Clinical application of the tension-stress effect for limb lengthening [J]. Clin orthop, 1990, 250: 8.
3. Ilizarov GA. The tension-stress ecffet on the genesis and growth of tissues: I. The influence of stability on fixation and soft-tissue preservation [J]. Clin Orthop, 1989, 238: 249-281.
4. McCarthy JG, Schreiber J, Karp NS, et al. Lengthening the human mandible by gradual distraction [J]. Plast Reeonstr Surg, 1992, 89 (l): 1-8.
5. McCarthy JG. Distraction osteogenesis on reconstruction of jaw bone defects [J]. Plast Reconstr Surg, 1992, 12 (3): 239-246.
6. Hatefi S, Alizargar J, Roux F L, et al. Review of physical stimulation techniques for assisting distraction osteogenesis in maxillofacial reconstruction applications [J]. Medical Engineering & Physics, 2021 (1).
8. Verlinden C, van de Vijfeijken, S. E. C. M, Tuinzing D B, et al. Complications of mandibular distraction osteogenesis for developmental deformities: a systematic review of the literature [J]. International Journal of Oral and Maxillofacial Surgery, 2014, 44 (1): 44-49.
9. P. P. J. M. van der Plas, Streppel M, Pullens B, et al. Feeding and swallowing outcomes following mandibular distraction osteogenesis: an analysis of 22 non-isolated paediatric cases – ScienceDirect [J]. International Journal of Oral and Maxillofacial Surgery, 2022; 51 (7): 892-899.
10. Ozturk S A, Malkoc S, Yolcu U, et al. Three-dimensional soft tissue evaluation after rapid maxillary expansion and mandibular midline distraction osteogenesis [J]. Angle Orthodontist, 2021, 91 (5): 634-640.
11. Hongyu Chen, Guanhui Cai, Xiaolei Ruan, et al. Bone-targeted bortezomib increases bone formation within Calvarial trans-sutural distraction osteogenesis. Bone, 2023 (169): 116677.
12. Humphries L S, Zapatero Z D, Vu G H, et al. Ten Years of Posterior Cranial Vault Expansion by Means of Distraction Osteogenesis: An Update and Critical Evaluation [J]. Plastic and reconstructive surgery, 2022 (2): 150.
13. Califano L, Cortese A, Aupi A, et al. Mandibular lengthening by external distraction: An experimental study in the rabbit [J]. Oral Maxillofac Surg, 1994, 52: 1179-1183.
14. Li G, Bouxsein M L, Luppen C, et al. Bone consolidation is enhanced by rhBMP-2 in a rabbit model of distraction osteogenesis [J]. Journal of Orthopaedic Research, 2010, 20 (4): 779-788.
15. Pleun P. J. M. van der Plas, Joosten K, Wolvius E B, et al. Mandibular distraction to correct severe non-isolated mandibular hypoplasia: The role of drug-induced sleep endoscopy (DISE) in decision making [J]. International Journal of Pediatric Otorhinolaryngology, 2022, 152: 110968.
16. Karp NS, McCarthy JG, Schreiber JS, et al. Membranous bone lengthening: a serial histological study [J]. Ann Plast Surg, 1992, 29 (l): 2-7.
17. Komuro Y, Takato T, Harii K, et al. The histological analysis of distraction osteogenesis of the mandible in rabbits [J]. Plast Reconstr Surg, 1994, 94 (l): 152-159.
18. Lan S M, Snke M, Chris P. Microstructure evolution of young sea ice from a Svalbard fjord using micro-CT analysis [J]. Journal of Glaciology, 2022 (269): 68.
19. Karjalainen, V. -P. Kestila, I. Finnila, M. A. Folkesson, E. Turkiewicz, A. Onnerfjord, P. Hughes, V. Tjonstand, J. Englund, M. Saarakkala, S. Quantitative three-dimensional collagen orientation analysis of human meniscus posterior horn in health and osteoarthritis using micro-computed tomography [J]. Osteoarthritis and cartilage, 2021, 29 (5).
20. Valenzuela T F, Iaizzo P A. Post-procedure micro-CT analyses of coronary artery stenting in left main vessels of reanimated and perfusion-fixed human hearts [J]. BioMedical Engineering OnLine, 2023, 22 (1).
21. Peng Li, Longduo Shen, Jia Li, Rui Liang, Weidong Tian, Wei Tang. Optimal design of an individual endoprosthesis for the reconstruction of extensive mandibular defects with finite element analysis [J]. J cranio-maxillo-facial surg, 2014, 42 (1): 73-8.
22. I. G L, N. T J, C. H I. The Simultaneous Use of Spring-Mediated Cranioplasty and Distraction Osteogenesis as a Safe Modality for Multi-Suture Craniosynostosis [J]. The Journal of craniofacial surgery, 2022 (5): 33.