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1Department of Research Methodology and Biostatistics, University of Medical Sciences and Technology, Khartoum, Sudan
2Department of Microbiology, Faculty of Medical Laboratory Sciences, University of Medical Sciences and Technology, Khartoum, Sudan
3Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
4Department of Dairy Science and Technology, Sudan University of Science and Technology, Khartoum, Sudan
Commiphora myrrha, is an ancient plant which exhibits an antiseptic and anti-inflammatory effect for mouth and it has been traditionally used for its antimicrobial properties in oral health care. This study assessed the impact of C. myrrha on two bacteria Streptococcus mutans and Lactobacillus spp. involved in dental caries. Three samples of Streptococcus mutans bacteria were collected randomly from patients with dental caries in Khartoum dental teaching hospital, while Lactobacillus spp. isolates were obtained from fermented milk. Disc and well diffusion methods were used to test the effect of four concentrations (100, 50, 25 and 12.5 mg/ml) of Myrrh volatile oil, extracted by hydro-distillation technique. Ampicillin, Vancomycin and Ciprofloxacin were used as control positive. The phytochemical analysis of C. myrrha oil was carried out using Gas Chromatography Mass Spectroscopy technique. The finding of this study revealed that the four concentrations of essential oil were effective on S. mutans with the largest inhibition zone (18.7± 0.6 mm) through the well diffusion method and inhibition zone of 14.00 mm with disc diffusion method, regardless the two methods; the inhibition zones were recorded at 100 mg/ml, with Minimum Bactericidal Concentration (MBC) at 3.125 mg/ml. On the other hand, Lactobacillus spp. bacteria was found to be sensitive to three concentrations of oil (100, 50 and 25 mg/ml) and resistance to lowest concentration (12.5 mg/ml), the MBC found to be 25 mg/ml. The results of GC-MS revealed the presence of 48 compounds, the highest percentage were; Benzofuran (29.13%) followed by Cyclohexane (19.88%) and 1,3-Diphenyle-1,2-butanediol (15.17%) respectively. Myrrh oil is effective on both S. mutans and Lactobacillus spp. Hence, it is a potential antibacterial product of interest in dental caries.
Myrrh, Essential Oil, Cavities, Streptococcus mutans, Lactobacillus spp.
Reem Izzeldien, Sondos Ibn Ouf, Ayat Ahmed Alrasheid, Zawahir Abu Elbashar, Mounkaila Noma. (2023). Impact of Commiphora myrrha on Bacteria (Streptococcus mutans and Lactobacillus spp.) Related to Dental Caries. International Journal of Dental Medicine, 9(2), 38-44. https://doi.org/10.11648/j.ijdm.20230902.12
Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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