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Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis

Objective: To explore the application of self-made distractors to construct a unilateral DO model of rabbit mandible and evaluate the microstructure of bone specimens. Method: Designing a linear dual arm distractor and performing DO implantation surgery on 5 rabbits under local anesthesia. Stretching the mandible at rate of 1.0mm/day, 2 times/day for 10 days. After fixation for 3 months, taking mandibular specimens for gross observation, Micro-CT imaging and HE histological examination to analyze the bone density, maturity and three-dimensional structure of the new bone. Result: Except for one animal that died of unknown cause, the right mandibular body of the other animals was extended by about 10mm. The traction gap is completely filled with bright and red new bone with clear contours; Micro-CT reconstruction of three-dimensional bone images clearly shows high bone density in the gaps, with trabeculae arranging along the direction of distraction; HE staining shows that the bone trabeculae were connected into woven and layered bones, with a large number of concentric arranged bone plates surrounding the central canal to form the Haversian system, and osteoblasts proliferated actively. Conclusion: The use of Micro-CT and HE staining proves the success of the unilateral DO model of rabbit mandible using a self-made distractor, providing animal experiment for various biological factors and drug assisted methods to promote efficient DO in the later stage, and for its safe and effective clinical application.

Distraction Ssteogenesis, Mandible, Micro-CT, Three-Dimensional Structure

Li Peng, Xiao Xin, He Chuying, Song Jing, Long Jie. (2023). Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. International Journal of Dental Medicine, 9(1), 23-27.

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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